A Novel Method to Chromatographically Resolution Of Sulphonamides By Vapour-Programmed Thin-Layer Chromatography

Authors

Ala Eldin Choukaife - Loai  Aljerf

Abstract

In pharmaceutical sulphonamides, vapour-programmed thin-layer chromatography (VP-TLC) was implemented using Silica Gel GF254 in the separation and characterisation of sulphonamides. The outcomes of  this method have been compared with thin layer chromatographic (TLC) process. 15 chemical substances were resolved in a neutral and alkali media. Results are far from optimal. With ether-methanol (90: 10)  the first seven substances are reasonably separated but the remaining eight all show about the same migration. Furthermore, it should be noticed that only about 3/5 of the plate is utilised for the spread. Unsaturated  chambers did not improve the separation significantly. Only sulphamethoxypyridazine and sulphisoxazole did not separate under these conditions, but the other components are well separated and migrated  throughout the whole lane. It should be noted that the VP-systems described are suitable for two-dimensional development. Thus, it can be concluded that VP-TLC is highly effective in the analysis of sulphonamides

Keywords

Thin layer chromatographic; Sulphamethoxypyridazine; Pharmaceutical analysis; Anaesthetics; Amphoteric

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